Real-Time and In Situ Evaluation of Phycocyanin Concentration in Spirulina platensis Cultivation System by Using Portable Raman Spectroscopy

Spirulina platensis can synthesize a large amount of phycocyanin, which had been developed as health food. At the same time, absorb nitrogen and phosphorus in wastewater provide for its own growth. Here, we studied optimal supply production process. For first 405 nm portable Raman spectrometer was used to estimate phycocyanin content real-time industrial applications. We obtained three characteristic peaks through density functional theory combined with home-built spectrometer, were 1272, 1337, 1432 cm − 1 . There good linear correlation between sum peak intensities PCL concentration (y = 18.887x + 833.530, id="M2"> R 2 = 0.890 ). The least squares support vector machine model based on results coefficient prediction 0.907 residual predictive deviation 3.357. decision-making integration effluent treatment references Spirulina-based biorefinery